Digital Microscope

什么是数字显微镜？

Adigital microscope是一种成像工具，它使用带有放大镜的内置相机，而不是目镜，并在监视器上显示实时图像。Keyence数字显微镜设计为适用于所有行业的各种不同的应用。这种多功能系统主要用于实验室或质量保证和研发部门。

科斯数码显微镜VHX-7000

数字显微镜 - 镜头和图像传感器系统

数字显微镜s make observation fast and convenient. They use zoom style lenses, a combination of multiple individual lenses, that make the system easier to operate compared to other microscopes: simply turn the magnification ring on the lens to adjust magnification.

The performance of a lens is affected by these 3 main factors:景深, numerical aperature, and aberration. The KEYENCE digital microscope has a 20 times larger depth-of-field compared to conventional optical microscopes due to its naturally smaller aperture, resulting in objects always being in full focus within a large tolerance range. The ability to focus light(numerical aperature)增强显微镜镜片的质量，并保持像差，投影误差最小。

Image captured on a conventional optical microscope (left) and Digital microscope (right)

Keyence数字显微镜使用内置CMOS图像传感器that can convert light passing through the lens into digital data and display that information in the form of an image. When the light hits the photodiodes on the surface, electron hole pairs are generated and produce electrical signs that are converted into digital form, via a transfer electrode, allowing the data taken from the CMOS sensor to be displayed on the digital microscope monitor.

Signal path from the image sensor to the VHX-7000 digital microscope display

PhotoDiodes无法识别颜色，结果，将滤色器或棱镜内置在显微镜中以再现它们。数字显示器的质量最终取决于颜色和屏幕分辨率。颜色分辨率由X和Y轴中的像素数确定。

数字显微镜: typical screen resolutions

Seevolume 1我们的‘茵特罗德女士’uction to Digital Microscopy guide for more on the lens and image sensor system.

图像捕获数字显微镜的性能

The颜色分辨率or color depth provides the number of intensity levels that can be displayed of the 3 primary colors: red, green, and blue. For typical digital microscopes, the resolution is 8-bit with 256 x 256 x 256 color values, which allows over 16 million colors to be diplayed.

最新的keyency数字显微镜具有HDR功能，可以捕获16位RGB图像数据，比以前的模型产生256倍。自动颜色，亮度，对比度和纹理优化允许实际显示对象的细节，而大量亮度级别会减少眩光并增强具有低对比度的区域。

Images of a connector (100x) and a stepped stencil (2000x) in 8 and 16 bit captured with the KEYENCE digital microscope

Seevolume 2我们对数字显微镜指南的介绍，更多关于成像捕获性能。

深度数码显微镜的深度组成

景深is the area in which the object being observed is in full focus, even outside the focal point. For typical optical microscopes, the depth-of-field decreases when magnification increases. With the focusing technology of the KEYENCE digital microscope, this restriction is overcome and full focused observation is achievable even at high magnification. The depth composition function slightly moves the lens focus, extracting and stitching focused pixels, displaying a fully focused image on the digital microscope monitor across an entire field of view at any magnification.

在每个级别的聚焦像素一起提取和拼接允许keyence数字显微镜创建整个视野的完全聚焦的图像

Seevolume 3我们对数字显微镜指南的介绍，更多关于深度组成。

Illumination options for a digital microscope

数字显微镜具有专用光源，这允许根据观察距离和镜头视场来实现最佳光分布。在数字显微镜下，许多照明技术可用于各种各样的表面条件和目标物体形状。

Punctiform illumination, known ascoaxial illumination, is the best option for highly reflective surfaces or when observing the texture of an object, as it uses a half mirror to allow all the reflected light to pass through the lens in order to optimize the image display.

使用漫反射照明应观察到镜子，加工金属或塑料，以防止图像中的高光泽度，使得数字显微镜可以从各个方向照射物体。

Schematic diagram of selected illumination techniques for digital microscopes: coaxial illumination (left) and diffuse illumination

360°侧照明适配器允许从不同角度照明并表示可以优化对象上的投影和压痕的显示。Keyence数字显微镜也具有部分照明，以帮助使这些预测和压痕更清晰可见。

Finally, transparent objects can be best observed from below using传输光照射. Apolarizing filter还允许将反射的光滤出，以避免图像中的镜面反射。

Seevolume 4我们对数字显微镜指南的介绍，以了解数字显微镜上有哪些照明选项。